Article
The pmr gene, encoding a Ca2+-ATPase, is required for calcium and manganese homeostasis and normal development of hyphae and conidia in Neurospora crassa.
Eukaryotic cell - 1 Nov 2012
Bowman Barry J, Abreu Stephen, Johl Jessica K, Bowman Emma Jean
Abstract excerpt
The pmr gene is predicted to encode a Ca(2+)-ATPase in the secretory pathway. We examined two strains of Neurospora crassa that lacked PMR: the Δpmr strain, in which pmr was completely deleted, and pmr(RIP), in which the gene was extensively mutated. Both strains had identical, complex phenotypes. Compared to the wild type, these strains required high concentrations of calcium or manganese for optimal growth and...
Topics
- Calcium
- Cell Membrane
- Chlorides
- Culture Media
- Endoplasmic Reticulum
- Fungal Proteins
- Gene Expression Regulation, Enzymologic
- Genes, Fungal
- Golgi Apparatus
- Green Fluorescent Proteins
- Homeostasis
- Hyphae
- Manganese