Techniques used to define human MHC antigens: restriction fragment length polymorphisms.

doi:10.1016/0165-2478(91)90195-g

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Article

Techniques used to define human MHC antigens: restriction fragment length polymorphisms.

Immunology letters - 1 Jul 1991

Middleton D

Abstract excerpt

Polymorphisms within the HLA-DRB1, -DRB3, -DQB1 and -DQ A1 genes are detectable using restriction fragment length polymorphism (RFLP) analysis. DNA is isolated from EDTA-treated blood or from spleen or lymph nodes. The DNA is digested to completion with the restriction endonuclease TaqI and resol...

Topics

Alleles
DNA
DNA Probes
Genotype
HLA Antigens
HLA-DQ Antigens
HLA-DR Antigens
Humans
Kidney Transplantation
Polymorphism, Restriction Fragment Length

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