Article
A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors.
Proceedings of the National Academy of Sciences of the United States of America - 5 Jul 2006
Colot Hildur V, Park Gyungsoon, Turner Gloria E, Ringelberg Carol, Crew Christopher M, Litvinkova Liubov, Weiss Richard L, Borkovich Katherine A, Dunlap Jay C
Abstract excerpt
The low rate of homologous recombination exhibited by wild-type strains of filamentous fungi has hindered development of high-throughput gene knockout procedures for this group of organisms. In this study, we describe a method for rapidly creating knockout mutants in which we make use of yeast recombinational cloning, Neurospora mutant strains deficient in nonhomologous end-joining DNA repair, custom-written...
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