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Article

Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies

14 Jan 2008

Abstract excerpt

BACKGROUND: Due to the limited RNA amounts from endomyocardial biopsies (EMBs) and low expression levels of certain genes, gene expression analyses by conventional real-time RT-PCR are restrained in EMBs. We applied two preamplification techniques, the TaqMan(R) PreAmp Master Mix (T-PreAmp) and a multiplex preamplification following a sequence specific reverse transcription (SSRT-PreAmp). RESULTS: T-PreAmp...

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PMID: 18194512
DOI: 10.1186/1471-2199-9-3

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