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Article

Genotyping of single Cryptosporidium oocysts in sewage by semi-nested PCR and direct sequencing.

Water research - 1 Jul 2006

Hashimoto Atsushi, Sugimoto Hitomi, Morita Shigemitsu, Hirata Tsuyoshi

Abstract excerpt

This study describes an approach for genotyping individual Cryptosporidium oocysts obtained from sewage. We isolated single immunofluorescent assay (IFA)-stained Cryptosporidium oocysts from sewage concentrate using glass capillary pipettes and inverted epifluorescence microscopy. Each isolated Cryptosporidium oocyst was analyzed by semi-nested PCR for the 18S rRNA gene and direct sequencing of the PCR products....

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Identifiers

PMID: 16790257
DOI: 10.1016/j.watres.2006.04.038

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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