Article
Molecular fingerprinting of Cryptosporidium oocysts isolated during water monitoring.
Applied and environmental microbiology - 1 Aug 2006
Nichols Rosely A B, Campbell Brian M, Smith Huw V
Abstract excerpt
We developed and validated a PCR-based method for identifying Cryptosporidium species and/or genotypes present on oocyst-positive microscope slides. The method involves removing coverslips and oocysts from previously examined slides followed by DNA extraction. We tested four loci, the 18S rRNA gene (N18SDIAG and N18SXIAO), the Cryptosporidium oocyst wall protein (COWP) gene (STN-COWP), and the dihydrofolate...
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