Article
The detection of Cryptosporidium and the resolution of mixtures of species and genotypes from water.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 1 Apr 2013
Ruecker Norma J, Matsune Joanne C, Lapen David R, Topp Edward, Edge Thomas A, Neumann Norman F
Abstract excerpt
Environmental concentrations of Cryptosporidium require molecular assays with ultra-sensitive detection limits and which provide critical information on genetic diversity within the genus, a feat particularly challenging from a diagnostics point of view. In this study, the performance of repetitive nested PCR-RFLP and limiting template dilution repetitive nested PCR-RFLP were assessed for their ability to detect...
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