Article
Schwann cell preparation from single mouse embryos: analyses of neurofibromin function in Schwann cells.
Methods in enzymology - 1 Jan 2006
Ratner Nancy, Williams Jon P, Kordich Jennifer J, Kim Haesun A
Abstract excerpt
The study of peripheral nerve function in development and disease can be facilitated by the availability of cultured cells that faithfully mimic in vivo Schwann cell growth, maturation, and differentiation. We have developed a method to establish purified mouse Schwann cell culture from a single embryo at embryonic day 12.5 (E12.5) to define the abnormalities in Schwann cells caused by loss of the...
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