Article
A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves.
Scientific reports - 23 Aug 2016
Andersen Natalia D, Srinivas Shruthi, Piñero Gonzalo, Monje Paula V
Abstract excerpt
We herein developed a protocol for the rapid procurement of adult nerve-derived Schwann cells (SCs) that was optimized to implement an immediate enzymatic dissociation of fresh nerve tissue while maintaining high cell viability, improving yields and minimizing fibroblast and myelin contamination. This protocol introduces: (1) an efficient method for enzymatic cell release immediately after removal of the...
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