Article
A highly specific real‐time RT‐PCR method for the quantitative determination of CK‐19 mRNA positive cells in peripheral blood of patients with operable breast cancer
17 May 2006
Abstract excerpt
The aim of the present study was to decrease the incidence of false positives and to better characterize marginally cytokeratin-19 (CK-19) mRNA positive peripheral blood samples from patients with early stage breast cancer. A new set of highly specific primers for CK-19, which avoids amplification of contaminating genomic DNA, was designed and evaluated to improve the specificity and sensitivity of the previously...
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