Article
Validation of a low-cost human papillomavirus genotyping assay based on PGMY PCR and reverse blotting hybridization with reusable membranes.
Journal of clinical microbiology - 1 Oct 2011
Estrade C, Menoud P-A, Nardelli-Haefliger D, Sahli R
Abstract excerpt
The genotyping of human papillomaviruses (HPV) is essential for the surveillance of HPV vaccines. We describe and validate a low-cost PGMY-based PCR assay (PGMY-CHUV) for the genotyping of 31 HPV by reverse blotting hybridization (RBH). Genotype-specific detection limits were 50 to 500 genome equivalents per reaction. RBH was 100% specific and 98.61% sensitive using DNA sequencing as the gold standard (n = 1,024...
Topics
- Adult
- Clinical Laboratory Techniques
- Female
- Genotype
- Humans
- Membranes
- Middle Aged
- Nucleic Acid Hybridization
- Papillomaviridae
- Papillomavirus Infections
- Polymerase Chain Reaction