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Article

Human Keratocytes Cultured on Amniotic Membrane Stroma Preserve Morphology and Express Keratocan

24 Nov 2003

Abstract excerpt

PURPOSE: To develop a new method of expanding human corneal keratocytes in serum while maintaining their characteristic morphology and keratocan expression. METHODS: Human keratocytes were isolated from central corneal buttons by digestion in 1 mg/mL of collagenase A in DMEM and seeded on plastic or the stromal matrix of human amniotic membrane (AM) in DMEM with different concentrations of FBS. On confluence,...

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PMID: 14638709
DOI: 10.1167/iovs.03-0484

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