Use of a signature nucleotide sequence of hepatitis C virus for detection of viral RNA in human serum and plasma

doi:10.1128/jcm.29.11.2528-2534.1991

Article

Use of a signature nucleotide sequence of hepatitis C virus for detection of viral RNA in human serum and plasma

1 Nov 1991

Abstract excerpt

The nucleic acid sequence of the putative 5'-untranslated (5PUT) region of hepatitis C virus (HCV), determined for samples obtained from a variety of geographic origins, was found to be over 98% conserved among all isolates. On the basis of this signature sequence for HCV, a viral RNA assay was developed by using cDNA synthesis with reverse transcriptase, followed by polymerase chain reaction (PCR). The new assay...

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