Article
A second-generation method of genotyping hepatitis C virus by the polymerase chain reaction with sense and antisense primers deduced from the core gene.
Journal of virological methods - 1 Mar 1996
Okamoto H, Kobata S, Tokita H, Inoue T, Woodfield G D, Holland P V, Al-Knawy B A, Uzunalimoglu O, Miyakawa Y, Mayumi M
Abstract excerpt
A second-generation method of genotyping hepatitis C virus (HCV) was developed by the polymerase chain reaction (PCR) with sense as well as antisense primers deduced from the core gene. HCV RNA specimens extracted from sera were reverse-transcribed and amplified with universal primers in the first round of PCR to obtain fragments of 433 base pairs representing nucleotides 319-751. In the second round of PCR,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.