Article
Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes.
Journal of biochemistry - 1 Sept 1991
Nakamura Y, Oda K, Nakada S
Abstract excerpt
In order to establish cell lines which complement the growth of temperature-sensitive (ts) mutants of influenza virus, three RNA polymerase and nucleoprotein (NP) genes each linked to the mouse mammary tumor virus LTR were cloned into the bovine papillomavirus vector DNA. After co-transfection of mouse C127 cells with these recombinant plasmids, a cell line, clone 76, in which the expression of the three...
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