cis-acting ompF mutations that result in OmpR-dependent constitutive expression.

Abstract excerpt

OmpR and EnvZ differentially control the transcription of the major outer membrane porin genes, ompF and ompC, in Escherichia coli in response to the osmolarity of the medium. We have previously provided evidence that OmpR works both positively and negatively at the ompF promoter to give the characteristic switch from OmpF to OmpC production with increasing osmolarity. Here, we describe the isolation of...

Topics

• Bacterial Outer Membrane Proteins
• Base Sequence
• DNA, Bacterial
• DNA-Binding Proteins
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Genes, Bacterial
• Molecular Sequence Data
• Mutation
• Nucleic Acid Conformation
• Phenotype