Article
The CDC8 gene product is required for transformation with episomal and integrative plasmids in Saccharomyces cerevisiae.
Current genetics - 1 Sept 1991
Lecka-Czernik B, Zuk J
Abstract excerpt
The product of the yeast CDC8 gene (thymidylate kinase), which is required for chromosomal, mitochondrial and 2 mu plasmid replication, also participates in plasmid transformation processes in S. cerevisiae. The thermosensitive cdc8-1 mutant strain was transformed with episomal pDQ9 and integrative pDQ9-1 plasmids both of which carry the CDC8 gene. The results suggest that thymidylate kinase is essential for the...
Topics
- Chromosomes, Fungal
- Genes, Fungal
- Mutation
- Nucleoside-Phosphate Kinase
- Plasmids
- Recombination, Genetic
- Saccharomyces cerevisiae
- Temperature
- Transformation, Genetic