Article
A new system for amplifying 2 microns plasmid copy number in Saccharomyces cerevisiae.
Molecular microbiology - 1 Jun 1991
Unternährer S, Pridmore D, Hinnen A
Abstract excerpt
The yeast 2 microns plasmid is found in the nucleus of almost all Saccharomyces cerevisiae strains. Its replication is very similar to that of chromosomal DNA. Although the plasmid does not encode essential genes it is stably maintained in the yeast population and exhibits only a small, though de...
Topics
- DNA Ligases
- DNA Replication
- DNA, Fungal
- Gene Amplification
- Genes, Fungal
- Kinetics
- Mutation
- Plasmids
- Restriction Mapping
- Saccharomyces cerevisiae
- Temperature
- beta-Galactosidase
