Article
Pharmacological chaperone activity of SR49059 to functionally recover misfolded mutations of the vasopressin V1a receptor.
The Journal of biological chemistry - 26 May 2006
Hawtin Stuart R
Abstract excerpt
Pharmacological chaperones represent a new class of ligand with the potential to facilitate the delivery of misfolded, but still active, G-protein-coupled receptors to the cell surface. Using transfected HEK 293T cells, treatment with a nonpeptide antagonist, SR49059, dramatically increased ( approximately 60-fold) the surface expression of a misfolded, nonfunctional and intracellularly localized vasopressin...
Topics
- Amino Acid Sequence
- Cell Membrane
- Hormone Antagonists
- Humans
- Immunohistochemistry
- Indoles
- Ligands
- Microscopy, Confocal
- Molecular Chaperones
- Molecular Sequence Data
- Mutation