Article
A hydrophobic protein sequence can override a nuclear localization signal independently of protein context.
Molecular and cellular biology - 1 Oct 1991
van Zee K, Appel F, Fanning E
Abstract excerpt
Simian virus 40 T antigen is specifically targeted to the nucleus by the signal Pro-Lys-Lys-128-Lys-Arg-Lys-Val. We have previously described the isolation of a simian virus 40 T-antigen mutant, 676FS, which retains a wild-type nuclear localization signal but fails to accumulate properly in the nucleus and interferes with the nuclear localization of heterologous proteins. Here we report that the hydrophobic...
Topics
- Amino Acid Sequence
- Antigens, Polyomavirus Transforming
- Cell Line
- Cell Nucleus
- Fluorescent Antibody Technique
- Molecular Sequence Data
- Mutation
- Nuclear Proteins
- Protein Sorting Signals
- Recombinant Fusion Proteins