Article
Systematic replacement of lysine with glutamine and alanine in Escherichia coli malate synthase G: effect on crystallization.
Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Dec 2005
Anstrom David M, Colip Leslie, Moshofsky Brian, Hatcher Eric, Remington S James
Abstract excerpt
Two proposals recommend substitution of surface lysine residues as a means to improve the quality of protein crystals. In proposal I, substitution of lysine by alanine has been suggested to improve crystallization by reducing the entropic cost of ordering flexible side chains at crystal contacts. In proposal II, substitution of lysine by residues more commonly found in crystal contacts, such as glutamine, has...
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