Article
Arg305 of Streptomyces L-glutamate oxidase plays a crucial role for substrate recognition.
Biochemical and biophysical research communications - 20 Jan 2012
Utsumi Tomohiro, Arima Jiro, Sakaguchi Chika, Tamura Takashi, Sasaki Chiduko, Kusakabe Hitoshi, Sugio Shigetoshi, Inagaki Kenji
Abstract excerpt
Recently, we have solved the crystal structure of L-glutamate oxidase (LGOX) from Streptomyces sp. X-119-6 (PDB code: 2E1M), the substrate specificity of which is strict toward L-glutamate. By a docking simulation using L-glutamate and structure of LGOX, we selected three residues, Arg305, His312, and Trp564 as candidates of the residues associating with recognition of L-glutamate. The activity of LGOX toward...
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