A combination of RNase H (rnh) and recBCD or sbcB mutations in Escherichia coli K12 adversely affects growth.

doi:10.1007/BF00273933

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Article

A combination of RNase H (rnh) and recBCD or sbcB mutations in Escherichia coli K12 adversely affects growth.

Molecular & general genetics : MGG - 1 Jul 1991

Itaya M, Crouch R J

Abstract excerpt

Colony forming ability of Escherichia coli strains carrying the rnh-339::cat mutant allele is strongly dependent on the recBCD and sbcB genes. A mutation inactivating either the RecBCD nuclease or exonuclease I (sbcB) is sufficient to restrict severely the efficiency of plating of strains carryin...

Topics

Alleles
Blotting, Southern
Chloramphenicol O-Acetyltransferase
DNA, Bacterial
Endoribonucleases
Escherichia coli
Exodeoxyribonuclease V
Exodeoxyribonucleases
Genes, Bacterial
Genes, Lethal
Mutation
Recombination, Genetic
Ribonuclease H
Temperature

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