Article
Screening for candidate genes involved in tolerance to organic solvents in yeast.
Applied microbiology and biotechnology - 1 Jun 2006
Matsui Ken, Hirayama Takashi, Kuroda Kouichi, Shirahige Katsuhiko, Ashikari Toshihiko, Ueda Mitsuyoshi
Abstract excerpt
Saccharomyces cerevisiae mutant strain, KK-211, isolated from serial culture in medium containing isooctane showed an extremely higher tolerance to the hydrophobic organic-solvents, which are toxic to yeast cells compared to the wild-type parent strain, DY-1. To detect genes that are related to this tolerance, a DNA microarray analysis was performed using mRNAs isolated from strains DY-1 and KK-211. Fourteen...
Topics
- Culture Media
- Genes, Fungal
- Microarray Analysis
- Mutation
- Octanes
- Organic Chemicals
- Saccharomyces cerevisiae
- Solvents
