Use of inosine-containing oligonucleotide primers for enzymatic amplification of different alleles of the gene coding for heat-stable toxin type I of enterotoxigenic Escherichia coli.

doi:10.1128/aem.57.4.955-961.1991

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Article

Use of inosine-containing oligonucleotide primers for enzymatic amplification of different alleles of the gene coding for heat-stable toxin type I of enterotoxigenic Escherichia coli.

Applied and environmental microbiology - 1 Apr 1991

Candrian U, Furrer B, Höfelein C, Lüthy J

Abstract excerpt

A method which employs the polymerase chain reaction (PCR) to identify Escherichia coli strains containing the estA gene was developed. This gene codes for heat-stable enterotoxin type I. The use of an inosine-containing pair of amplification primers allowed the amplification of a specific 175-bp DNA fragment from several different estA alleles. The amplified fragments were identified and distinguished by...

Topics

Alleles
Animals
Bacterial Toxins
Base Sequence
DNA Restriction Enzymes
DNA, Bacterial
Electrophoresis, Agar Gel
Enterotoxins
Enzyme-Linked Immunosorbent Assay
Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Escherichia coli Infections

Escherichia coli Proteins

Humans

Inosine

Molecular Sequence Data

Oligodeoxyribonucleotides

Polymerase Chain Reaction

Templates, Genetic

Source

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Identifiers

PMID: 1647751
DOI: 10.1128/aem.57.4.955-961.1991
PMCID: PMC182829

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1647751-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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