Article
PCR assay for detection and differentiation of K88ab(1), K88ab(2), K88ac, and K88ad fimbrial adhesins in E. coli strains isolated from diarrheic piglets.
Folia microbiologica - 1 Jan 2005
Holoda E, Vu-Khac H, Andrasková S, Chomová Z, Wantrubová A, Krajnák M, Pilipcinec E
Abstract excerpt
Primers were designed and prepared and conditions were determined for PCR detection and differentiation of enterotoxigenic E. coli bacterial strains isolated from diarrheic pigs. Primers K88/1 and K88/2 are 25 bp oligomers that correspond to a region of genes encoding one of serological variants of the K88 antigen (K88ab(1), K88ab(2), K88ac or K88ad). A positive result of PCR is an amplificate of 792 bp in size...
Topics
- Animals
- Antigens, Bacterial
- DNA Primers
- DNA Restriction Enzymes
- Diarrhea
- Escherichia coli
- Escherichia coli Infections
- Escherichia coli Proteins
- Fimbriae Proteins
- Genetic Variation
- Polymerase Chain Reaction
- Slovakia
- Swine
- Swine Diseases