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Article

Detection of Shigella in Feces Using DNA Amplification

1 Jun 1990

Abstract excerpt

A rapid diagnostic method employing a polymerase chain reaction procedure (PCR) was used to identify Shigella and enteroinvasive Escherichia coli. This procedure amplified a region of the invasive-associated locus (ial) from a crude DNA extract of feces. A synthetic 21-base oligonucleotide corresponding to the ial gene sequence was shown to specifically hybridize only with enteroinvasive E. coli (EIEC) strains...

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PMID: 2189008
DOI: 10.1093/infdis/161.6.1252

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