Article
[Study on the molecular epidemiology of diarrhea caused by enterotoxigenic Escherichia coli, using polymerase chain reaction with multiple primer pairs].
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi - 1 Aug 1997
Cai Q, Wang W, Chen Y C
Abstract excerpt
A Polymerase Chain Reaction (PCR) method has been developed for detecting Enterotoxigenic Escherichia coli (ETEC). Three different sets of oligonucleotide primer were simultaneously used to amplify the enterotoxin genes of heat-labile (LTh) and heat-stable (STIa and STIb) enterotoxins of ETEC. These primers amplified the 627, 240 or 169 base pair DNA fragments from LTh, STIa and STIb genes of the reference ETEC...
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