Article
[Improving thermostability of Aspergillus niger phytase by elongation mutation].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Nov 2005
Chen Hui, Wang Hong-Ning, Yang Wan-Shen, Zhao Hai-Xia, Wu Qi, Shan Zhi
Abstract excerpt
The phytase gene phyA(m) from Aspergillus niger N25 was recombined into E. coli expression vector pET-30b(+). Recombined at expression vectors pET30b-FphyA(m) was served as a template to amplify phytase gene, and the PCR product named elongation mutation gene phyA(e) was expanded with a 13 amino...
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