Article
Improving phytase enzyme activity in a recombinant phyA mutant phytase from Aspergillus niger N25 by error-prone PCR.
Applied biochemistry and biotechnology - 1 Feb 2012
Liao Yan, Zeng Min, Wu Zhen-Fang, Chen Hui, Wang Hong-Ning, Wu Qi, Shan Zhi, Han Xue-Yi
Abstract excerpt
The mutant acid phytase (phyA ( m )) gene was modified by random mutagenesis to improve enzymatic activity by using an error-prone PCR (ep-PCR) strategy. The mutated gene was linearized and inserted into plasmid vector pPIC9K and transformed by electroporation into Pichia pastoris GS115. A single transformant, PP-NP(ep)-6A, showing the strongest phytase activity from among the 5,500 transformants, was selected...
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