Article
A functional leuABCD operon is required for leucine synthesis by the tyrosine-repressible transaminase in Escherichia coli K-12.
Journal of bacteriology - 1 Jun 1991
Vartak N B, Liu L, Wang B M, Berg C M
Abstract excerpt
In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was tightly linked to the leu operon (at 1.5 min), not to tyrB (at 92 min). Chromosomal fragments...
Topics
- Bacteriophages
- Base Sequence
- DNA Transposable Elements
- Escherichia coli
- Genes, Bacterial
- Genes, Viral
- Genotype
- Leucine
- Molecular Sequence Data
- Mutation
- Operon