Article
Inducible functions in hybrids of a Lyt-2+ BW5147 transfectant and the 2C CTL line.
Immunogenetics - 1 Jan 1992
Gu J J, Gottlieb P D
Abstract excerpt
Cytolytic activity and release of interleukin 2 (IL-2) were induced in Lyt-2-positive T-T cell hybrids by incubation with either concanavalin A or irradiated stimulator cells. Since hybrids of Lyt-2-positive class I-specific cytotoxic T lymphocytes (CTLs) with the fusable mouse thymoma cell line, BW5147, are invariably Lyt-2-negative, a derivative of BW5147 was produced by transfection which constitutively...
Topics
- Animals
- Antibodies, Monoclonal
- Antigens, Ly
- Cell Line
- Concanavalin A
- Flow Cytometry
- Gene Expression
- H-2 Antigens
- Hybrid Cells
- Interleukin-2
- Mice
- Mice, Inbred BALB C