Article
Biochemical and structural aspects of transiently and stably expressed mutant desmin in vimentin-free and vimentin-containing cells.
European journal of cell biology - 1 Jun 1992
Raats J M, Gerards W L, Schreuder M I, Grund C, Henderik J B, Hendriks I L, Ramaekers F C, Bloemendal H
Abstract excerpt
Using immunoelectron microscopy it is demonstrated that desmin subunits missing their complete carboxy-terminal domain are incapable of homopolymeric filament formation in vivo. Furthermore it is shown that, in vimentin-containing cells, desmin integrates into preexisting vimentin filaments resul...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Blotting, Western
- Cell Line
- Cloning, Molecular
- DNA Mutational Analysis
- Desmin
- Fluorescent Antibody Technique
- HeLa Cells
- Humans
- Intermediate Filaments
- Microscopy, Immunoelectron
- Molecular Sequence Data
- Mutation
- Plasmids
- Transfection
- Vimentin