Article
Assembly of amino-terminally deleted desmin in vimentin-free cells.
The Journal of cell biology - 1 Nov 1990
Raats J M, Pieper F R, Vree Egberts W T, Verrijp K N, Ramaekers F C, Bloemendal H
Abstract excerpt
To study the role of the amino-terminal domain of the desmin subunit in intermediate filament (IF) formation, several deletions in the sequence encoding this domain were made. The deleted hamster desmin genes were fused to the RSV promoter. Expression of such constructs in vimentin-free MCF-7 cells as well as in vimentin-containing HeLa cells, resulted in the synthesis of mutant proteins of the expected size....
Topics
- Amino Acid Sequence
- Animals
- Biopolymers
- Cell Line
- Chromosome Deletion
- Cricetinae
- Desmin
- Fluorescent Antibody Technique
- Humans
- Intermediate Filaments
- Keratins