Article
Systematic reverse genetics of transfer-DNA-tagged lines of Arabidopsis. Isolation of mutations in the cytochrome p450 gene superfamily.
Plant physiology - 1 Nov 1998
Winkler R G, Frank M R, Galbraith D W, Feyereisen R, Feldmann K A
Abstract excerpt
We have developed an efficient reverse-genetics protocol that uses expedient pooling and hybridization strategies to identify individual transfer-DNA insertion lines from a collection of 6000 independently transformed lines in as few as 36 polymerase chain reactions. We have used this protocol to...
Topics
- Arabidopsis
- Base Sequence
- Cytochrome P-450 Enzyme System
- DNA Primers
- DNA, Bacterial
- Multigene Family
- Mutation
- Phenotype
- Polymerase Chain Reaction