Article
Systematic reverse genetics of transfer-DNA-tagged lines of Arabidopsis. Isolation of mutations in the cytochrome p450 gene superfamily.
Plant physiology - 1 Nov 1998
Winkler R G, Frank M R, Galbraith D W, Feyereisen R, Feldmann K A
Abstract excerpt
We have developed an efficient reverse-genetics protocol that uses expedient pooling and hybridization strategies to identify individual transfer-DNA insertion lines from a collection of 6000 independently transformed lines in as few as 36 polymerase chain reactions. We have used this protocol to systematically isolate Arabidopsis lines containing insertional mutations in individual cytochrome P450 genes. In...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.