Article
Genetic dissection of transcriptional regulation by cDNA-AFLP.
The Plant journal : for cell and molecular biology - 1 Feb 2006
Vuylsteke Marnik, Daele Hilde, Vercauteren Annelies, Zabeau Marc, Kuiper Martin
Abstract excerpt
This study demonstrates that cDNA-AFLP is a powerful gel-based genome-scale transcript profiling technique to generate quantitative gene expression profiles for eQTL mapping. We used cDNA-AFLP to monitor the relative abundance of 912 transcripts across 50 Arabidopsis thaliana recombinant inbred lines. Estimates for heritability of cDNA-AFLP intensity polymorphisms were high, with a median of 0.30 and an...
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