Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Genetic characterization of CHO production host DG44 and derivative recombinant cell lines.

Biochemical and biophysical research communications - 24 Feb 2006

Derouazi M, Martinet D, Besuchet Schmutz N, Flaction R, Wicht M, Bertschinger M, Hacker D L, Beckmann J S, Wurm F M

Abstract excerpt

The dihydrofolate reductase-deficient Chinese hamster ovary (CHO) cell line DG44 is the dominant mammalian host for recombinant protein manufacturing, in large part because of the availability of a well-characterized genetic selection and amplification system. However, this cell line has not been studied at the cytogenetic level. Here, the first detailed karyotype analysis of DG44 and several recombinant...

Topics

Join the communities discussing this publication.

CHO Cells
Chromosome Aberrations
Chromosomes, Mammalian
Cricetinae
Cricetulus

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Green Fluorescent Proteins

Recombinant Proteins

Recombination, Genetic

Source

Identifiers

PMID: 16403443
DOI: 10.1016/j.bbrc.2005.12.111

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Genetic characterization of CHO production host DG44 and derivative recombinant cell lines.PMID 16403443Read ↗

A comparative genomic hybridization approach to study gene copy number variations among Chinese hamster cell lines.Semantic proximity Read ↗

Identification of transgene integration loci of different highly expressing recombinant CHO cell lines by FISHSemantic proximity Read ↗

Structural analysis of random transgene integration in CHO manufacturing cell lines by targeted sequencingSemantic proximity Read ↗

Growth Rate Changes in CHO Host Cells Are Associated with Karyotypic HeterogeneitySemantic proximity Read ↗

Transcriptomic variation between different Chinese hamster ovary cell lines.Semantic proximity Read ↗

Microevolutionary dynamics of eccDNA in Chinese hamster ovary cells grown in fed-batch cultures under control and lactate-stressed conditionsSemantic proximity Read ↗

Optimization of cell line development in the GS-CHO expression system using a high-throughput, single cell-based clone selection systemSemantic proximity Read ↗

Biallelic gene knockouts in Chinese hamster ovary cells.Semantic proximity Read ↗

Chromosome-mediated gene transfer with resultant expression and integration of the transferred genes in eukaryotic cells.Semantic proximity Read ↗

Cytogenetic analysis of chimeric antibody-producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressureSemantic proximity Read ↗

Chromosomal destabilization during gene amplification.Semantic proximity Read ↗

Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressureSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.