Article
HIV-1 reverse transcriptase gene 103K/N and 184M/V combinations in tandem: detection and quantification of HIV-1 populations in the CD45RO+ T-cell compartment by a double-ARMS real-time PCR assay.
Journal of acquired immune deficiency syndromes (1999) - 1 Feb 2006
Mohey Rajesh, Tolstrup Martin, Jørgensen Louise B, Møller Bjarne K, Black Finn T, Kjems Jørgen, Obel Niels
Abstract excerpt
BACKGROUND: The proviral HIV-1 reverse transcriptase gene for the 103K/N and 184M/V combinations were studied in tandem. The CD45RO T (memory) cell compartment was investigated. METHODS: A new double-ARMS (amplification refractory mutation system) real-time polymerase chain reaction assay was developed to detect and quantify 4 populations (103K-184M, 103K-184V, 103N-184M, and 103N-184V) in the CD45RO T-cell...
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