Article
Promoter cloning and characterisation of the transcriptional regulation of the human thyrostimulin A2 subunit.
Molecular and cellular endocrinology - 21 Dec 2005
Breous Ekaterina, Wenzel Achim, Loos Ulrich
Abstract excerpt
The novel heterodimeric glycoprotein hormone thyrostimulin consists of two unique subunits, A2 and B5. To understand its yet unknown transcriptional regulation, we characterised the 3.1-kb immediate 5'-flanking region of the human A2 gene localised on chromosome 11q13. In transient transfection assays this sequence exhibited promoter activity, which could be confined to nucleotides -506 to -347 relative to the...
Topics
- Animals
- Cell Line
- Cloning, Molecular
- Codon
- Electrophoretic Mobility Shift Assay
- Exons
- Gene Deletion
- Gene Expression Regulation
- Glycoproteins
- Humans
- Mice
- Mutation
- Peptide Hormones
- Promoter Regions, Genetic
- Regulatory Elements, Transcriptional
- Silencer Elements, Transcriptional
- Transcription Factors
- Transcription, Genetic