Article
Amino acid changes in Drosophila alphaPS2betaPS integrins that affect ligand affinity.
The Journal of biological chemistry - 24 Feb 2006
Bunch Thomas A, Helsten Teresa L, Kendall Timmy L, Shirahatti Nikhil, Mahadevan Daruka, Shattil Sanford J, Brower Danny L
Abstract excerpt
We developed a ligand-mimetic antibody Fab fragment specific for Drosophila alphaPS2betaPS integrins to probe the ligand binding affinities of these invertebrate receptors. TWOW-1 was constructed by inserting a fragment of the extracellular matrix protein Tiggrin into the H-CDR3 of the alphavbeta3 ligand-mimetic antibody WOW-1. The specificity of alphaPS2betaPS binding to TWOW-1 was demonstrated by numerous tests...
Topics
- Amino Acid Motifs
- Animals
- Cytoplasm
- Dose-Response Relationship, Drug
- Drosophila
- Drosophila Proteins
- Edetic Acid
- Integrin alpha Chains
- Integrin alphaVbeta3
- Integrins
- Ligands
- Manganese
- Models, Molecular