Article
Contractile rabbit aortic smooth muscle cells in culture. Preparation and characterization.
American journal of hypertension - 1 Jun 1992
Shirinsky V P, Birukov K G, Sobolevsky A V, Vedernikov Y P, Posin EYa, Popov E G
Abstract excerpt
Investigations were conducted to determine whether rabbit aortic smooth muscle cells (SMC) reproduce their essential in situ features in culture. Enzymatically isolated cells in culture were compared with their in situ state in terms of myosin and caldesmon isoform expression, sensitivity to Ca(2+)-mobilizing agonists, and contractility. Protein marker expression was assessed by electrophoresis and quantitative...
Topics
- Animals
- Aorta
- Calcium
- Calmodulin-Binding Proteins
- Cells, Cultured
- Electrophoresis, Polyacrylamide Gel
- Histamine
- Immunoblotting
- Isomerism
- Models, Biological
- Muscle Contraction
