Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1.

doi:10.1101/gad.6.7.1280

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1.

Genes & development - 1 Jul 1992

Gartner A, Nasmyth K, Ammerer G

Abstract excerpt

The FUS3 and KSS1 kinases are components of the pheromone-dependent signal transduction pathway in yeast. We show that FUS3 and KSS1 become rapidly phosphorylated after pheromone treatment. Similar to mammalian MAP kinases, this modification occurs at two amino acids of FUS3, threonine-180 and tyrosine-182. A mutation introduced at either position results in complete loss of function in vivo. Amino acid...

Topics

Alleles
Amino Acid Sequence
Base Sequence
DNA, Fungal
Enzyme Activation
Fungal Proteins
Immunoblotting
MAP Kinase Kinase Kinases
Mitogen-Activated Protein Kinases
Molecular Sequence Data
Pheromones

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phosphorylation

Protein-Tyrosine Kinases

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Signal Transduction

Threonine

Tyrosine

Source

Open in PubMed

Identifiers

PMID: 1628831
DOI: 10.1101/gad.6.7.1280

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1628831-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1.PMID 1628831Read ↗

A role for autophosphorylation revealed by activated alleles of FUS3, the yeast MAP kinase homolog.Semantic proximity Read ↗

Signaling in the yeast pheromone response pathway: specific and high-affinity interaction of the mitogen-activated protein (MAP) kinases Kss1 and Fus3 with the upstream MAP kinase kinase Ste7.Semantic proximity Read ↗

The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5.Semantic proximity Read ↗

Constitutive activation of the Saccharomyces cerevisiae transcriptional regulator Ste12p by mutations at the amino-terminus.Semantic proximity Read ↗

Saccharomyces cerevisiae Ste5 is important for induction and substrate specificity of Fus3 MAP kinase in the pheromone signaling pathway.Semantic proximity Read ↗

Dynamic ubiquitination of the mitogen-activated protein kinase kinase (MAPKK) Ste7 determines mitogen-activated protein kinase (MAPK) specificity.Semantic proximity Read ↗

Control of MAPK signaling specificity by a conserved residue in the MEK-binding domain of the yeast scaffold protein Ste5.Semantic proximity Read ↗

Fus3-regulated Tec1 degradation through SCFCdc4 determines MAPK signaling specificity during mating in yeast.Semantic proximity Read ↗

Activation of an MAP kinase cascade leads to Sir3p hyperphosphorylation and strengthens transcriptional silencing.Semantic proximity Read ↗

Strains isogenic to S288C used in the yeast genome sequencing programme carry a functional KSS1 gene.Semantic proximity Read ↗

A conserved docking site in MEKs mediates high-affinity binding to MAP kinases and cooperates with a scaffold protein to enhance signal transmission.Semantic proximity Read ↗

Pheromone-dependent destruction of the Tec1 transcription factor is required for MAP kinase signaling specificity in yeast.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.