Article
Defined genetic events associated with the spontaneous in vitro transformation of ElA/Ras-expressing human IMR90 fibroblasts.
Carcinogenesis - 1 Feb 2006
Mason Douglas X, Keppler Daniel, Zhang Jun, Jackson Tonya J, Seger Yvette R, Matsui Seiichi, Abreo Fleurette, Cowell John K, Hannon Gregory J, Lowe Scott W, Lin Athena W
Abstract excerpt
In contrast to rodent cells, normal human fibroblasts are generally resistant to neoplastic transformation in vitro. Here, we report the derivation and characterization of a spontaneously transformed cell line from normal human IMR90 fibroblasts transduced with E1A and Ras oncogenes. Unlike the parental, non-tumorigenic E1A/Ras-expressing IMR90 cells, these spontaneously transformed cells displayed aberrant...
Topics
- Adenovirus E1A Proteins
- Cell Culture Techniques
- Cell Transformation, Neoplastic
- DNA-Binding Proteins
- Fibroblasts
- Gene Expression Regulation
- Genes, myc
- Genes, ras
- Genetic Predisposition to Disease
- Humans
- Telomerase
- Transduction, Genetic