Article
Cytomegalovirus glycoprotein B sequence variation among Japanese bone marrow transplant recipients.
Intervirology - 1 Jan 1997
Wada K, Mizuno S, Kato K, Kamiya T, Ozawa K
Abstract excerpt
Glycoprotein B (gB) of human cytomegalovirus (CMV) is the major target protein of neutralizing antibodies, and four variant types are known. A previously reported polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method was modified by eliminating the cell culture step and amplifying a CMV fragment (corresponding to a.a. 441-450 of gB) by nested PCR. DNA was extracted from the sera...
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