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Isolation, culture, and characterization of embryonic cell lines from vitrified sheep blastocysts

1 Jan 2005

Abstract excerpt

This study was conducted to isolate, to culture, and to characterize embryonic cell lines from in vitro produced vitrified sheep blastocysts. Embryos were produced and vitrified at the expanded blastocyst stage. Ten inner cell masses arising from day 6-7 blastocysts were isolated by immunosurgery, disaggregated, and cultured onto mitomocin-C-inactivated mouse STO fibroblasts (MIF). After 5 or 6 days of culture...

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PMID: 16206132
DOI: 10.1002/mrd.20378

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