Article
Culture of mouse amniotic fluid-derived cells on irradiated STO feeders results in the generation of primitive endoderm cell lines capable of self-renewal in vitro.
Cells, tissues, organs - 1 Jan 2013
Babic Aleksandar M, Jang Sunyoung, Nicolov Eugenia, Voicu Horatiu, Luckey Chance J
Abstract excerpt
The cells present in amniotic fluid (AF) are currently used for prenatal diagnosis of fetal anomalies but are also a potential source of cells for cell therapy. To better characterize putative progenitor cell populations present in AF, we used culture conditions that support self-renewal to determine if these promoted the generation of stable cell lines from AF-derived cells (AFC). Cells isolated from E11.5 mouse...
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