Article
Cloning and molecular characterization of a human recombinant IgG Fab binding to the Tat protein of human immunodeficiency virus type 1 (HIV-1) derived from the repertoire of a seronegative patient.
Molecular immunology - 1 Mar 2006
Mancini Nicasio, Perotti Mario, Carletti Silvia, Canducci Filippo, Sampaolo Michela, Clementi Massimo, Burioni Roberto
Abstract excerpt
A study aiming at cloning and characterizing natural antibodies to human immunodeficiency virus type 1 (HIV-1) targets is described. In particular, we report the molecular cloning of a Fab molecule binding the HIV-1/Tat protein from a seronegative patient. The Fab was characterized for its binding specificity and investigated in regards to its molecular structure. Furthermore, to evaluate the role played by the...
Topics
- Cloning, Molecular
- Gene Products, tat
- HIV Antibodies
- HIV Seronegativity
- HIV-1
- Humans
- Immunity, Innate
- Immunoglobulin Fab Fragments
- Immunoglobulin G
- Mutation
- Protein Binding
- Recombinant Proteins
- tat Gene Products, Human Immunodeficiency Virus