Article
Active site topology of Saccharomyces cerevisiae lanosterol 14 alpha-demethylase (CYP51) and its G310D mutant (cytochrome P-450SG1).
The Journal of biological chemistry - 5 Jul 1992
Tuck S F, Aoyama Y, Yoshida Y, Ortiz de Montellano P R
Abstract excerpt
Incubation of phenyldiazene (PhN = NH) with lanosterol 14 alpha-demethylase, a cytochrome P-450 enzyme (CYP51) that oxidatively removes the 14 alpha-methyl group of lanosterol, results in the appearance of a 478-nm band indicative of phenyl-iron complex formation. In situ oxidation of the phenyl-...
Topics
- Binding Sites
- Catalysis
- Chromatography, High Pressure Liquid
- Cytochrome P-450 Enzyme System
- Ferricyanides
- Imines
- Mutation
- Myoglobin
- Oxidation-Reduction
- Oxidoreductases
- Protein Conformation
- Saccharomyces cerevisiae