Cloning and functional analysis of a beta-tubulin gene from a benomyl resistant mutant of Aspergillus parasiticus.

doi:10.1016/s0378-1119(96)00382-4

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Article

Cloning and functional analysis of a beta-tubulin gene from a benomyl resistant mutant of Aspergillus parasiticus.

Gene - 5 Dec 1996

Wu T S, Skory C D, Horng J S, Linz J E

Abstract excerpt

A genomic DNA library prepared from a benomyl resistant strain of Aspergillus parasiticus was screened with a Neurospora crassa beta-tubulin gene probe. A unique A. parasiticus genomic DNA fragment, thought to carry a mutant beta-tubulin gene (benr), was isolated. Two plasmids, pYT1 and pYTPYRG, carrying the putative benr gene or benr plus a second selectable marker (pyrG), respectively, were used to transform a...

Topics

Aspergillus
Bacteriophage lambda
Benomyl
Blotting, Southern
Cell Division
Cloning, Molecular
DNA Probes
Drug Resistance
Fungal Proteins
Fungicides, Industrial
Genetic Markers
Mutagenesis
Mutation
Plasmids
Sequence Analysis
Transformation, Genetic
Tubulin

Source

Open in PubMed

Identifiers

PMID: 8982061
DOI: 10.1016/s0378-1119(96)00382-4

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