Article

Early detection of Cryphonectria parasitica by real-time PCR

2019-01-01

Abstract excerpt

The development and validation of a real-time PCR method for the detection of Cryphonectria parasitica in bark tissues is described. The selected region in the genome was a fragment of the internal transcribed spacer region. The DNA extraction and PCR conditions were optimized to be routinely applicable to fresh and dried bark. The sensitivity of the assay allowed the detection of 2 fg of genomic DNA, equivalent t...

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Literature Corpus work: c4c5e7b5-c329-50a1-b471-4f99d4cf158c
DOI: 10.1007/s10658-018-1538-0

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